A new technique brings CRISPR-mediated gene editing together with spatial transcriptomics and imaging to study the ...

Immune checkpoint inhibitors (ICIs), drugs that release the immune system’s “brakes,” are meant to expose cancer to the full force of the immune system’s attack. But in most cancer patients, ICIs ...

Analyzing complex gene networks can be as easy as one, two, three. One: Introduce CRISPR-based perturbations to large numbers of cells. Two: Measure the effects of these perturbations with single-cell ...

Whitehead Institute Member Jonathan Weissman and collaborators used their single-cell sequencing tool Perturb-seq on every expressed gene in the human genome, linking each to its job in the cell and ...

"We present several improvements to this approach, which together lay the groundwork for performing Perturb-seq screens at larger scale and with combinatorial perturbations," Adamson said. The first ...

Collaborative research defines a novel approach to understanding how certain proteins called transcription factors determine which genetic programs will drive cell growth and maturation. The method, ...

Researchers combine pooled CRISPR screening with single-cell RNA sequencing to enable high-resolution analysis of transcriptional profiles on a large scale. Diagram of CRISPR-seq (4). Genetic screens ...

Joseph Replogle, an MD-PhD student in Jonathan Weissman’s lab at the Whitehead Institute/MIT (MA, USA), explains how the technology he worked to develop – direct capture Perturb-seq – has ...